YE6144 is a prototypical interferon regulatory factor 5 (IRF5) inhibitor. YE6144 selectively suppresses IRF5 activity through inhibition of IRF5 phosphorylation[1].

In Vitro

YE6144 (1 or 3 μM; 30 min) inhibits the phosphorylation of IRF5 in both human PBMCs and mouse splenocytes^[1].
YE6144 (0-10 μM; 30 min) inhibits the production of type I IFNs with an IC₅₀ of approximately 0.09 μM in human HC PBMCs^[1].

Western Blot Analysis^[1]

In Vivo

YE6144 (40.0 mg/kg; s.c.; once) suppresses the progression of mouse systemic lupus erythematosus^[1].

[1]. Ban T, et al. Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease. Nat Commun. 2021 Jul 19;12(1):4379.  [Content Brief]