| TAC03290 |
CY7-Amidite |
|
98%+ |
| TAC03325 |
Cy7.5 NHS Ester |
|
98%+ |
| TAC00710 |
Cyanine 5 NHS ester |
|
98%+ |
| TAC00739 |
DAF-2 DA |
|
98%+ |
| TAC03360 |
digoxigenin NHS Ester |
|
98%+ |
| TAC00188 |
DiI |
|
98%+ |
| TAC01064 |
DiR |
|
98%+ |
| TAC03310 |
dSpacer CE Phosphoramidite |
|
98%+ |
| TAC03312 |
dT-Alkynyl Amidite |
|
98%+ |
| TAC03287 |
dT-NH2-Amidite |
|
98%+ |
| TAC03302 |
fluorescein d T |
|
98%+ |
| TAC00355 |
Fluorescein-5-maleimide |
|
98%+ |
| TAC03306 |
HEX Amidite |
|
98%+ |
| TAC03260 |
Infrared fluorescence excitation dye 806 |
|
98%+ |
| TAC04178 |
IRDye 700DX NHS Ester |
|
98%+ |
| TAC01138 |
JC-1 |
|
98%+ |
| TAC03304 |
JOE Amidite |
|
98%+ |
| TAC00974 |
Lyso-Tracker Green |
|
98%+ |
| TAC00299 |
Mito-tracker green |
|
98%+ |
| TAC00007 |
Mitosox red |
MitoSOX Red is a novel fluorescent probe that specifically targets mitochondria in living cells with cell membrane permeability. MitoSOX Red enters the mitochondria, it is oxidized by superoxide, but not by other ROS or RNS generating systems. MitoSOX Red then binds to intramitochondrial nucleic acids, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. Additionally, Superoxide dismutase (SOD) prevents the oxidation of MitoSOX Red.
Excitation/emission wavelength: 510/580 nm. Storage: Keep away from light.
Usage method
1. Preparation of MitoSOX Red working solution
1.1 Preparation of storage solution Using 13 μ Dilute 50 with anhydrous DMSO μ G MitoSOX Red to prepare 5 mM of stock solution. Note: MitoSOX Red storage solution is recommended to be stored in dark at -20 ℃ or -80 ℃ after packaging.
1.2 Preparation of working fluid Dilute the storage solution with preheated serum-free cell culture medium or PBS to prepare 1-10 μ MitoSOX Red working fluid for M. Note: Please adjust the concentration of MitoSOX Red working solution according to the actual situation and prepare it as needed. 2. Cell staining (suspended cells)
2.1 Centrifuge and collect cells, add PBS and wash twice, each time for 5 minutes. Cell density at 1 × 106/mL.
2.2 Add 1 mL of MitoSOX Red working solution and incubate at room temperature for 5-30 minutes.
2.3 400 g, centrifuge for 3-4 minutes, discard the supernatant.
2.4 Add PBS to wash the cells twice, each time for 5 minutes. After resuspending cells with 1 mL of serum free medium or PBS, observe using a fluorescence microscope or flow cytometry.
3. Cell staining (attach parietal cell)
3.1 Culture parietal cell on sterile cover slides.
3.2 Remove the cover glass from the medium and remove excess medium.
3.3 Add 100 μ L dye working solution, gently shake to completely cover the cells, and incubate for 5-30 minutes.
3.4 Remove the dye working solution, wash with culture medium 2-3 times, each time for 5 minutes, and observe using a fluorescence microscope or flow cytometry.
Saving conditions -Store in dark at 20 ℃ for one year matters needing attention
1. Please adjust the concentration and incubation time of MitoSOX Red working solution according to the actual situation.
2. This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, nor for food or medicine. For your safety and health, please wear laboratory clothes and disposable gloves for operation. |
98%+ |
